New 100bp Ladder Diluted and Functional

The new Invitrogen 100 base pair was tested during my R16 cycle course PCR.

The ladder can be seen here, along with third wash samples from six, nine, twelve, fifteen, and twenty cycles of PCR. As N58 RNA is slightly larger than 100 base pairs, its location just above the lowest ladder band is an indication of its effectiveness. Multiple 20 uL aliquots were created and placed in the -20 freezer. The stock ladder solution, previously diluted by Brad, was placed in the ladders and primers freezer box, distinguished by red tape.

1 comment:

Nia_Fernandez said...

Does the figure look blurry because of the website or is this how it looked when you took it? You want to avoid blurry images for your reports!