Biotinylation of Targets

Hey guys, so I was about to start the selection for my target this semester but realized that it's not a guarantee whether my target (KRAS) is biotinylated or not. This is obviously important because I want to know whether I should perform a bead or filter selection for this target. But this question probably applies to everyone's targets, to what extent should we trust the labeling on the freezer for biotinylated and non-biotinylated?

Thanks,

Ryan

Questions regarding Proposal

I noticed that in the Abstract, Introduction and Background, Experimental Design ect., AND Budget it asks us to include information in regards to ordering. I feel like it is kind of redundant, but then again, I've never had to write a proposal. Also, from what I've gathered thus far, it seems as though the information necessary for the budget is not going to fill up an ENTIRE page. Feedback? Thanks!

-Vivian Esparza

Radiation Training Woes

Hi Gwen,

Leta, Melissa, Dustin, and I tried to go to radiation training class scheduled today at 9:30, but we were told we could not attend this class because it was only designated for senior physics majors (?!). There is another class on September 12th, but several of us have conflicts with this time.

Stephanie Tutak