Specific Aim 1: Selection of RNA aptamers against S100A4
S100A4 promotes cell progression through angiogenesis and allows tumor neovascularization and cell progression of cancer cells (Sherbet 2008). Therefore decreased levels of this protein correlate to decreased metastasis. Selection of RNA aptamers against S100A4 will decrease metastasis of cancer and tumor cells and since S100A4 also protects from apoptotic stimuli may also induce apoptosis in these malignant cells.
Hua J., D. Chen, H. Fu, R. Zhang, W. Shen, S. Liu, K. Sun, and X. Sun. 2009. Short hairpin RNA–mediated inhibition of S100A4 promotes apoptosis and suppresses proliferation of BGC823 gastric cancer cells in vitro and in vivo. Cancer Letters, 292 (1): 41-47.
Sherbet, G.V. 2008. Metastasis promoter S100A4 is a potentially valuable molecular target for cancer therapy. Cancer Letters, 280(1): 15-30.
Stoltenburg, R., C. Reinemann, and B. Strehlitz. SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomolecular Engineering 24 (4): 381-403.