tag:blogger.com,1999:blog-1195222727010537305.post3792949694333609756..comments2023-08-03T07:13:32.291-05:00Comments on Aptamer Project Site - 10% APS for Everyone: Nucleic Aptamer Selection Against S100A4 for Prevention of Cancer MetastasisAptamer Staffhttp://www.blogger.com/profile/11584531632318072295noreply@blogger.comBlogger11125tag:blogger.com,1999:blog-1195222727010537305.post-73246015963687521872012-04-11T13:41:33.742-05:002012-04-11T13:41:33.742-05:00i love this i blog im doing my post-doc on aptamer...i love this i blog im doing my post-doc on aptamers!Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-84595949478162840042010-10-13T17:44:59.515-05:002010-10-13T17:44:59.515-05:00To perform your calculations:
Since your company...To perform your calculations: <br /><br />Since your company did not provide you with preferred resuspension volume nor final concentration we used<br /><br />10 ul = 100 pmol for your calculations<br /><br />13,800 g/mol<br /><br />0.00002g * (mol/ 13,800g) * 1e12 = 1449.28 pmol/ 100 pmol/ aliquot = <br />14.49 aliquots<br /><br />14.49 aliquots * (10 ul/ aliquot) =<br />145 ul 1X TrisNia_Fernandezhttps://www.blogger.com/profile/06524075862801694775noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-31492520250042985812010-10-13T16:34:29.146-05:002010-10-13T16:34:29.146-05:00I wrote Roger an e-mail checking on this target.I wrote Roger an e-mail checking on this target.Brad Hallhttps://www.blogger.com/profile/10516353314281930175noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-54732054354344092862010-09-07T13:59:12.530-05:002010-09-07T13:59:12.530-05:00@Kamaxi, I think the level of background in this a...@Kamaxi, I think the level of background in this abstract is appropriate, and anything more should be in the actual proposal. <br /><br />@Stephanie, this target is actually quite expensive. I found it from Genway, <a href="http://www.genwaybio.com/product_info.php?products_id=46724" rel="nofollow">Cat# 10-663-46724</a>, 20ug for $165. That means you only get 1449pmol. If you are really set on this protein, you can work with it. If you are set on cancer prevention, then work with another student on their target or find another target on your own. Other students are working with S100 related targets (see Kamaxi and Emma) and Amanda is working with CXCL1.Brad Hallhttps://www.blogger.com/profile/10516353314281930175noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-45020694639372126632010-09-07T13:03:46.094-05:002010-09-07T13:03:46.094-05:00You have a lot of interesting information about yo...You have a lot of interesting information about your protein! It provides a nice background and leads up to your specific point. <br /><br />Maybe you can provide some examples of things that have been done in the past using your protein and their results.Kamaxi Patelhttps://www.blogger.com/profile/01582781021778628618noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-36933721411299167772010-09-06T23:25:53.413-05:002010-09-06T23:25:53.413-05:00Gwen and Nia: Both of those I believe have sources...Gwen and Nia: Both of those I believe have sources - I have to double check if I just neglected to cite them in paragraph - they should be in the bibliography. Thanks for the heads up though!<br /><br />Mimi: Hmm, you make an good point, I definitely need to continue research on the protein - thanks for the heads up!Stephanie Philiphttps://www.blogger.com/profile/02905869490572196260noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-22305998587586684672010-09-01T16:15:54.023-05:002010-09-01T16:15:54.023-05:00"Chromosomal rearrangements and overexpressio..."Chromosomal rearrangements and overexpression of this protein have been implicated in the motility and metastasis of cancer through interactions with cytoskeletal constituents." -- > source?<br /><br />It seems to encompass a wide range of issues. It may be better to focus the purpose some more. <br /><br />It has very thorough protein information!!Nia_Fernandezhttps://www.blogger.com/profile/06524075862801694775noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-84352354593009728622010-09-01T13:47:02.703-05:002010-09-01T13:47:02.703-05:00This sounds like a good project. However, if the b...This sounds like a good project. However, if the body requires S100A4 for normal cell functions (motility and polymerization), how would you be able to regulate the amount of inhibition (by RNA aptamers) to the point where it becomes beneficial to the cell?Mimi Lehttps://www.blogger.com/profile/11138076535687920324noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-17244945685055520152010-09-01T11:36:05.914-05:002010-09-01T11:36:05.914-05:00This sounds like a really interesting project, we ...This sounds like a really interesting project, we have the same kind of end goal!Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-64608015416262259752010-09-01T10:41:18.938-05:002010-09-01T10:41:18.938-05:00Do you have a reference for this, "Therefore ...Do you have a reference for this, "Therefore decreased levels of this protein correlate to decreased metastasis"? Or - is this one of your hypotheses? Clarify this statement.Gwen Stovallhttps://www.blogger.com/profile/11462038982643107356noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-13023733696171480742010-09-01T10:37:32.400-05:002010-09-01T10:37:32.400-05:00Nice abstract. Sounds like a good project. Pleas...Nice abstract. Sounds like a good project. Please consider adding a sentence about how to obtain the protein.Gwen Stovallhttps://www.blogger.com/profile/11462038982643107356noreply@blogger.com