I will conduct two simultaneous bead based selections to develope an aptamer that binds to gag p24. However, in order to perform these bead based selections, the protein will have to be functionalized with a functional group that will allow it to be attached to the beads. Through these two selections, I will find multiple strong and weak binding aptamers to multiple functional regions on the p24 protein.
(1) Berthet-Colominas et al. (1999) Head-to-tail dimmers and interdomain flexibility revealed by the crystal structure of HIV-1 capsid protein (p24) complexed with a monoclonal antibody Fab. The EMBO Journal vol. 18 pp.1124-1136
(2) Gamble et al. (1997) Structure of Carboxyl-Terminal Dimerization Domain of the HIV-1 Capsid Protein. Science vol. 278 no. 5339, pp.849-853
Wikipedia sources used pervasive through the abstract:
Places to Find gag p24 (aka. p24 HIV-1):
RayBiotech- ($628 per .5 mg)