This space represents the ideas, views, opinions, projects and data of researchers within the Aptamer Stream of the Freshman Research Initiative, a program developed at the University of Texas at Austin. These are projects we currently have in the pipeline.
- We're not exclusive...we are SELECTIVE!
S100B is a Calcium binding
peptide commonly associated with disorders and injuries sustained to the CNS.
Its presence has been associated with medical conditions such as traumatic
brain injury, Alzheimer’s, Down Syndrome, dementia, and other neurological
disorders. Currently the levels of S100B mRNA found intra- and extra- cellular
are used to determine levels of astrocyte cell activity and or death in the brain
and spinal cord after injury (Figure 1).
When the blood brain barrier ,BBB, is
functioning normally, increases in the concentration of the protein in the
brain correlate with an increased immune response due to the creation of auto
antibodies against it, a trait commonly seen in disorders such as Alzheimer’s
An aptamer for S100B could not
only serve as a diagnostic tool for these CNS disorders but also serve as a way
to get in therapeutic drugs to help counteract the disruption of the blood
brain barrier once it has occurred. In
cases such as Alzheimer’s, the binding of an aptamer to the protein could help
to inhibit its interaction with glial cells by changing the shape so that it
can no longer bind to calcium ,receptors, or its own antibody.
Specific Aim 1: Selection of RNA
aptamers against S100B.
high affinity RNA aptamer could serve the purpose of inhibiting or detecting
S100B. By doing so, once levels of S100B reach certain levels the treatment,
detection, and possible prevention of CNS disorders associated with antibodies
against S100B could become more effective.
(Frances et al. 2003) Normally,
S100B is used in glial activation and for the CNS system on the brain side of
BBB in cells, such as the astrocyte cells pictured. Once the blood brain
barrier has been penetrated, excess amounts of S100B are secreted into the
blood allowing S100B to be used to test for the functionality of BBB. Levels on
the brain side of the barrier can also be used to test for neurological disorders
associated with high levels of S100B when the barrier is undisturbed.
currently in the lab so there are no additional costs associated with its
acquisition. It was provided by RayBiotech Inc. and is listed as catalog number 230-00002, Recombinant Human S100 Calcium
binding protein B. It can be purchased purchased for $326/100 ug with the
estimated cost per round totaling $7.44. RayBiotech Inc.’s contact information is http://www.raybiotech.com/ and (770)
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