RNA Bead Based Protocol Errors

Target Immobilization: Step 5. Denaturing should be @ 65C instead of 95C.

Eluting and Precipitating the RNA: Step 11. Resuspension should be in 10uL of diH2O not 20uL.

Reverse Transcription: Step 3. This step describes a component of the reaction mixture listed in step 4. I found this confusing when I was first perfroming selections. I think this step should be removed and the RNA template reactant should be added to the Rxn mixture list in step 4.

These are the errors that a few mentors and I knew of. If anyone else finds anything else please add a comment to this post.

(week 10 protocol)

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