Plan - week of March 21 - 27, 2011

One more time ...

Please retake the quiz, I'm sorry. The names didn't come across, so take it one more time, but enter in your name this time. I apologize for the inconvenience. Please take the quiz ASAP, so I can complete the target assignment.

Here's the plan for the week & how the targets will be assigned:

1. If you aren't enjoying the class and/or you want an "easy" target, then you'll stick with the lysozyme target. Lysozyme is a fun target! It'll make life a little bit easier for you & you get to see what it's all about to find an aptamer. Plus, you can hone your techniques and maybe feel a bit better about bench work in the long run.

Lysozyme is in the lab & you should be ready to run with these selections.

2. If you're unsure or somewhere in the middle, then you'll select against fibrinogen. This target has been selected against in the past, but an aptamer isn't necessarily almost guaranteed like with a lysozyme selection. Also - it's easier to understand the application of such an aptamer (i.e. easier than lysozyme aptamer application).

Fibrinogen in in the lab & you should be ready to run with these selections.

3. "Bring it!" If you're ready for a challenge, then you get maltose binding protein (MBP). Alex Miklos in the Ellington lab could use an aptamer to help him identify the "open" and "closed" forms of the protein. Here's what he said about it, "in pursuit of a surface-display system that I can use to evolve small-molecule binding proteins, an aptamer that would bind only to the closed form of a binding protein could prove to be useful (and probably publishable if nobody's done anything quite like this before)." Yes, the application of such an aptamer is a little bit more difficult to understand, so Alex will stop by & talk to us to get the point across.

The catch - MBP isn't ready yet. It won't be ready until the end of this week or early next week. So - if you get MBP, then you need to continue with your current selection until MBP is ready. Use the time to hone your techniques & learn some more about the protein.

Questions? Please post questions on the blog.

Thank you & see you this week!

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