Figure 1: Domain rearrangement in DENV protein E monomer in transition to trimer. a, Shows the tertiary structure of the DENV protein E
monomer in prefusion conformation. b, Shows the secondary structure and links between domains II and III of the protein. c, Shows the tertiary
structure of the DENV protein E monomer in postfusion trimer conformation. d, Shows the secondary structure and links between domains II and III.
Specific Aim 1: Selection of RNA aptamers against DENV protein E
DENV is a major public health concern that affects 112 countries.(1) DENV protein E is the virus' means of entrance in to host cells.(5) It has been shown that inhibition of viruses at the entry stage provides a route for treatment.(1) The selection of RNA aptamers that can bind to DENV protein E would provide molecules that can bind to and potentially inhibit DENV protein E. Recognition and/or inhibition of DENV protein E would provide a potential diagnostic or therapeutic tool.
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