Although there is already a renalase antibody, a renalase aptamer could be more beneficial in inhibiting its activity and detecting the levels of concentration of renalase. An aptamer is a specific nucleic acid sequence used to bind to a specific target molecule. It has a high specificity and can be selected to bind to any target since it can be obtained via in vitro. In contrast, an antibody requires an animal or a cell culture – making the aptamer more cost effective. Also, an aptamer can be denatured and renatured and still retain its binding ability, whereas an antibody cannot.
Specific aim 1: Selection of RNA aptamers against renalase
There are two reasons that an aptamer for renalase would be important. First off renalase is still a new protein in which further research can be done. Scientists are still proposing whether or not increased levels of renalase leads to kidney failure so by inhibiting the activity of this protein through use of an aptamer, more can be known about its functions. Secondly, a fluorescing aptamer can be beneficial by detecting the levels of renalase in the bloodstream. If levels are low, a dosage of renalase can be injected into the bloodstream which has proven to reduce blood pressure and risks of heart disease.
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8029, USA. “Renalase deficiency in chronic kidney disease, and its contribution to hypertension and cardiovascular disease” http://www.ncbi.nlm.nih.gov/pubmed/18277152; accessed Aug. 29, 2011.
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^ RNLS renalase, FAD-dependent amine oxidase. Entrez Gene. Accessed July 31, 2009.
Wang J, Qi S, Cheng W, Li L, Wang F, Li YZ, Zhang SP (December 2008). "Identification, expression and tissue distribution of a renalase homologue from mouse". Mol. Biol. Rep. 35 (4): 613–20. doi:10.1007/s11033-007-9131-1. PMID 17846919.
Li G, Xu J, Wang P, Velazquez H, Li Y, Wu Y, Desir GV (March 2008). "Catecholamines regulate the activity, secretion, and synthesis of renalase". Circulation 117 (10): 1277–82. doi:10.1161/CIRCULATIONAHA.107.732032. PMID 18299506. http://circ.ahajournals.org/cgi/pmidlookup?view=long&pmid=18299506.
Ghosh SS, Gehr TWB, Sica DA et al. Effect of renalase inhibition on blood pressure. J Am Soc Nephrology 2006; 17: 208A.
Desir GV, Wu Y, Wang P et al. Renalase deficiency increases sympathetic tone and causes hypertension. J Am Soc Nephrol 2008. Available at http://www.asn-online.org/education_and_meetings/renal_week/archives/
Zhao Q, Fan Z, He J, Chen S, Li H, Zhang P, Wang L, Hu D, Huang J, Qiang B, Gu D (August 2007). "Renalase gene is a novel susceptibility gene for essential hypertension: a two-stage association study in northern Han Chinese population". J. Mol. Med. 85 (8): 877
Desir GV, Li Y, Liu D et al. Downregulation of cardiac renalase expression in CKD, and protective effect of renalase in acute coronary syndrome. J Am Soc Nephrol 2007; 18: 149A.
Here is the link to my manuscript.
Here is my proposal.
Here is progress report #1.