Can you take a look at the following sequences (the clone check) and decide to re sequence some of them. Look if these have weird bands or look good and also nanodrop to determine concentration. Compare good sequence data to failed sequence data. Use the PCR product. Dilute the product 1:30 and resubmit 1ul of dilution + 10ul di H2O. Use the M13R (-24) primer for sequencing. I think there was a problem.
Good
16, 16, 11, 14, 16, 17, 24, 25, 29, 30, 33, 34, 37, 38, 39, 9, 8
Failed
15, 18, 26, 28, 32, 36, 1, 2, 4,
Let me know if the above observations show some reason why sequencing failed/worked
1 comment:
I shall do that this weekend and have it sent in by Monday since I will be in Houston this weekend.
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