tag:blogger.com,1999:blog-1195222727010537305.post384125525611214134..comments2023-08-03T07:13:32.291-05:00Comments on Aptamer Project Site - 10% APS for Everyone: Selection of RNA Aptamer against BPM-1027 for Diagnostic and Treatment Purposes of Burkholderia PseudomalleiAptamer Staffhttp://www.blogger.com/profile/11584531632318072295noreply@blogger.comBlogger7125tag:blogger.com,1999:blog-1195222727010537305.post-65068552067934111632010-09-07T10:59:12.666-05:002010-09-07T10:59:12.666-05:00Okay, for the pictures, just link to the picture o...Okay, for the pictures, just link to the picture online. I have corrected the text to remove the junk HTML formatting code. Please update the pictures or send the links to the ones you want via e-mail and I can post them for you.Brad Hallhttps://www.blogger.com/profile/10516353314281930175noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-90495694708557767522010-09-01T22:05:46.191-05:002010-09-01T22:05:46.191-05:00I have changed everything that you have suggested....I have changed everything that you have suggested. Thanks for your input.Katherine Lihttps://www.blogger.com/profile/01687911137413023998noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-66310259425257071732010-09-01T15:33:18.421-05:002010-09-01T15:33:18.421-05:00This protein is synthesized by Kate Brown, so when...This protein is synthesized by Kate Brown, so when we get it, it is functionalized with a 6X His Tag. The Oligo DT binds to everything so if the PVP works, it should show and difference between the + and the - selections, theoretically. We do - selections so I'm hoping to take out a lot of background using that. But also, after a few rounds, I will stop using the PVP and just do a few selections without it and then use a binding assay to determine how well PVP stops background binders. I hope to use the PVP as sort of a r0 pool starter that eliminates background to the tube and beads. This is just a theory I developed this summer.Katherine Lihttps://www.blogger.com/profile/01687911137413023998noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-50976727145392962962010-09-01T12:40:38.621-05:002010-09-01T12:40:38.621-05:00You mention the protein has a His tag. The native...You mention the protein has a His tag. The native protein does not contain a His tag. The recombinant protein purchased from the company contains a His tag ... <br /><br />What does oligo dT offer you? How will this help your selection?<br /><br />Also - are you concerned that you'll pull out ant-PVP aptamers? How did the Waterboer avoid this/Gwen Stovallhttps://www.blogger.com/profile/11462038982643107356noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-89218346399982486572010-09-01T09:28:21.532-05:002010-09-01T09:28:21.532-05:00Ok, Done. But Brad, now my pictures have become bl...Ok, Done. But Brad, now my pictures have become black holes....Katherine Lihttps://www.blogger.com/profile/01687911137413023998noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-43900504412021410882010-08-31T10:30:19.438-05:002010-08-31T10:30:19.438-05:00Oh, once you've corrected the paragraph, pleas...Oh, once you've corrected the paragraph, please let me know what changed in the comments so we can re-read...Brad Hallhttps://www.blogger.com/profile/10516353314281930175noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-30001249473681172682010-08-31T10:29:43.075-05:002010-08-31T10:29:43.075-05:00Of course the idea is good. It is a sponsored pro...Of course the idea is good. It is a sponsored projects. I want to address some wording and ideas though. Please address these within the text by either changing what you have written or providing more explanation within your abstract. Often this can be a few words or sentences. If I asked you to talk it through aloud, that would often be what you should write down. <br /><br />1.) How does a protein "Follow the regulations of" anything? Reword to suggest more scientifically specific domains appear to function similarly to type III secretion pathways and provide a reference. <br /><br />2. ) Nickel beads aren't a platform in themselves. Reword to suggest they are the preferred sieving process or something. <br /><br />3.) What does the Sim reference detailing the genome have to do with your first paragraph? Explain your thought process or remove.<br /><br />4.) I think paragraph two is a better intro paragraph. You may fit it in so the story goes from burk to disease to diagnostic protein target to aptamers.<br /><br />5.) Paragraph 3 seems like fluff without much substance. More rhetoric than scientific writing.<br /><br />6.) Reword the specific aim paragraph, second sentence starting with "It is thought..."<br /><br />7.) Move and reword the final sentence of specific aim 2. What other conditions? Be "specific". This can change later.Brad Hallhttps://www.blogger.com/profile/10516353314281930175noreply@blogger.com