tag:blogger.com,1999:blog-1195222727010537305.post3688148597217182547..comments2023-08-03T07:13:32.291-05:00Comments on Aptamer Project Site - 10% APS for Everyone: Danielle: Aptamer Selection Against Immunoglobulin E with the Possibility to Treat Allergic ResponseAptamer Staffhttp://www.blogger.com/profile/11584531632318072295noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-1195222727010537305.post-58708537181926467322012-09-13T12:48:31.210-05:002012-09-13T12:48:31.210-05:00Ankita - To answer your question, To my knowledge,...Ankita - To answer your question, To my knowledge, no I do not think it would be able to activate it, however I have a limited knowledge about the biology of antibodies. It is my understanding that this variable region is very specific to a particular antigen (in this case it would be an allergen that causes the reaction).<br /><br />What I am hoping to do throughout the process of my selection is to find something that will bind to the Fc region of the IgE and not the variable region, because we definitely don't want to select for something that is variable. The current method we have chosen is to order a full polyclonal IgE, so that there are multiple clones of IgE in the sample, not a monoclonal sample which would all have the same variable region. A better method would be to have just the Fc region in the selection process but due to limited budget its not feasible for us to procure that right now.Danielle Hoyerhttps://www.blogger.com/profile/05043835696733775261noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-81352307959925860652012-09-11T13:29:14.405-05:002012-09-11T13:29:14.405-05:00Dear Danielle,
Alright - let's try this again....Dear Danielle,<br />Alright - let's try this again. This time, I'll actually read your abstract & we'll wait to meet again to discuss the purchase your target (Fc only or IgE?) ...<br /><br />1. title - make more positive ... get rid of "possibilty". Reword title like you've already developed this.<br />2. remove "otherwise known as" from the 1st paragraph. Remember, this is a "dense" abstract.<br />3. ck capitalization of mast cell & basophils<br />4. put the citation # inside the punctuation<br />5. consider reorg ... introduce problem, narrow in on main culprit of prob, & then offer an aptamer sol'n<br />6. only include details that support your story in the abstract (elaboration may occur in the introduction)<br />7. get rid of if & might ...no weak words<br />8. specific aim #1 - identify an IgE (or Fc) aptamer<br />9. specific aim #2 - develop an aptamer therapeutic<br />10. budget - include amount or ug of target, include molecular weight, & include the cost per a round.<br />11. review reference #1 - does it have a page number? is all the info there?<br /><br />Looks good! We'll meet again tomorrow to hopefully purchase your target!<br />GwenGwen Stovallhttps://www.blogger.com/profile/11462038982643107356noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-74172941596517538772012-09-07T14:16:20.317-05:002012-09-07T14:16:20.317-05:00Dear Danielle,
Here are a few thoughts:
well, we d...Dear Danielle,<br />Here are a few thoughts:<br />well, we didn't get very far ...<br /><br />so - let's try to see if we can buy Human IgE Fc-region (only). Please continue searching. if you can't locate a vendor, then we need to come up w/ back-up plans ...<br />(1) select against IgE w/ variable region & see what we find. Maybe we'll luck out & select a Fc-region aptamer.<br />(2) ask Dr. Katy Brown (protein expert) to see if she knows where we could purchase it.<br />(3) mix-up the selection w/ IgE from different sources to vary the variable regions (love it!), while maintaining the Fc region.<br />(4) learn about antibodies<br /><br />Let's meet again. Please sign-up for another meeting.<br /><br />Thanks,<br />GwenGwen Stovallhttps://www.blogger.com/profile/11462038982643107356noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-47733485669314981672012-09-04T22:51:10.379-05:002012-09-04T22:51:10.379-05:00Very cool! Allergies are a big deal, especially ar...Very cool! Allergies are a big deal, especially around Austin. Question though, is there a possibility that an aptamer, instead of inhibiting, actually activates the site?Ankita B.https://www.blogger.com/profile/02010224983010908813noreply@blogger.com