tag:blogger.com,1999:blog-1195222727010537305.post2804661722699546634..comments2023-08-03T07:13:32.291-05:00Comments on Aptamer Project Site - 10% APS for Everyone: Progress for DENG SelectionAptamer Staffhttp://www.blogger.com/profile/11584531632318072295noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-1195222727010537305.post-40888610764700049122010-10-08T11:37:00.778-05:002010-10-08T11:37:00.778-05:00To holli's question, I am doing filter based s...To holli's question, I am doing filter based slx. As for the question about the two bands, I am not entirely sure. A sequence could have been lengthened by a strange primer effect. This particular sequence could have been replicated more readily by Taq. This could have lead to one particular length sequence that rose to a noticeable concentration.Michael Ledbetterhttps://www.blogger.com/profile/10907699454379743838noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-35158709711439703662010-10-06T16:18:11.710-05:002010-10-06T16:18:11.710-05:00I'm wondering why your round 2 cycle 15 has tw...I'm wondering why your round 2 cycle 15 has two bands? Any ideas?Nia_Fernandezhttps://www.blogger.com/profile/06524075862801694775noreply@blogger.comtag:blogger.com,1999:blog-1195222727010537305.post-76323051674293438162010-10-06T10:16:47.732-05:002010-10-06T10:16:47.732-05:00So far so good! Make sure to label this as the elu...So far so good! Make sure to label this as the elution. I had dropbox loading issues so I couldn't check, but are you doing bead or filter based slx? If you're doing bead, initially you might want to show some W3 data. Its good to see if your E amplifies sooner than weaker binding wash species.holli.duhonhttps://www.blogger.com/profile/15231237734464865212noreply@blogger.com