I know this picture is really hard to see, but the boxes show where the NTC bands were.
Pool: N58
Everything used were aliquots from the -20 fridge. I'm going to run another NTC using those same tubes, except for the PCR buffer and the reverse primer. I'll post on here when I run the gel and if I get a band.
Tianlu Ma

Oh wow yet another NTC amplification for N58!
ReplyDeleteI don't think it has anything to do with the RNA pool. I used the N34 pool and I still had amplification...
ReplyDelete